Transcriptional Regulation in Eukaryotes: Concepts, Strategies, and Techniques, Second Edition

Transcriptional Regulation in Eukaryotes: Concepts, Strategies, and Techniques, Second Edition

Subject Areas: Genetics and Genome Science; Molecular Biology; Biochemistry; Laboratory Manuals/Handbooks; Biophysics

By: Michael F. Carey, University of California, Los Angeles; Craig L. Peterson, University of Massachusetts Medical School, Worcester; Stephen T. Smale, University of California, Los Angeles

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Strategies for studying gene regulation mechanisms have changed dramatically over the past several years in light of the emergence of complete genome sequences for many organisms as well as the development of or improvements to technologies such as chromatin immunoprecipitation, RNA interference, microarrays, and proteomics.

The first edition of the highly successful Transcriptional Regulation in Eukaryotes, written by Michael Carey and Stephen Smale at UCLA, provided a comprehensive source of strategic, conceptual, and technical information for investigating the complexities of gene regulation at the level of transcription.

With the ever-increasing importance of genome data and the appearance of new and better techniques, the second edition of this book has added a third author, Craig Peterson at the University of Massachusetts Medical School. In addition to a new chapter on the in vitro analysis of chromatin templates for DNA-binding studies and transcription, this second edition has been extensively rewritten and updated to discuss new advances in the field and their impact on gene regulation mechanisms. The second edition retains the approach of the first in covering both the conceptual and practical aspects of how to study the regulation of a newly isolated gene and the biochemistry of a new transcription factor.

Transcriptional Regulation in Eukaryotes serves as both a powerful textbook and manual for advanced instruction in molecular biology which

supplements clearly written text with extensive illustrations
puts methods in the context of underlying theory
gives expert recommendations on experimental strategies
encourages creativity in investigative design
explains protocols for essential techniques step by step, with extensive advice on troubleshooting
provides the latest methods in use in the field

This important and unique book is essential reading for anyone pursuing the analysis of gene expression in model systems or disease states, providing underlying theory and perspective to the newcomer and the latest techniques to the expert.

Michael F. Carey, University of California, Los Angeles, was one of the authors of the first edition. His principal focus is biochemical mechanisms of gene activation and silencing, specifically the mechanism by which genes are controlled synergistically by upstream activators. He also studies the biochemistry of transcription on chromatin templates and the epigenetic control of gene expression.

Craig L. Peterson, University of Massachusetts Medical School, Worcester, concentrates on research that explores how chromosome structure influences nuclear processes and regulates genomic stability. He especially focuses on chromatin remodeling enzymes and the dynamics of chromatin fibers and their role in DNA repair, transcription, and replication.

Stephen T. Smale, University of California, Los Angeles, was also one of the authors of the first edition. His research focus is gene regulation during inflammatory and innate immune responses, with an emphasis on the mechanisms by which chromatin structure selectively regulates genes that promote inflammation. He also studies the regulation of immune system development by transcription factors and tumor suppressor proteins.

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